IAP-Like Protein 2, Blocking Peptide (ILP 2, Inhibitor of Apoptosis Protein)

Synthetic peptide (TGYEARLITFGT) corresponding to amino acids 2 to 13 of human ILP-2 (1).\n\nApoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family including IAP, XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibits speci?c proteases. A novel member in the IAP protein family was recently identi?ed and designated ILP-2 for IAP-like protein-2 (1). ILP-2 has high homology to ILP-1, but is encoded by a distinct gene that is solely expressed in testis of tested normal human tissues (1). ILP-2, unlike ILP-1, has no inhibitory effect on Fas and TNF induced apoptosis, but potently inhibits apoptosis induced by overexpression of Bax or by coexpression of caspase-9 with Apaf-1. ILP-2 interacts with the processed caspase-9. These results suggest that ILP-2 is a novel IAP family member with restricted speci?city for caspase-9.\n\nApplications: Suitable for use in ELISA and Western Blot. Other applications not tested.\n\nStorage and Stability: Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile 25% glycerol and store at -20 degrees C. May be stored at 4 degrees C for short-term only. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I0400P

Size

50ug

Applications

ELISA, WB

Form

Supplied as a lyophilized powder.

Purity

Purified 60-70%

References

1. Richter BW, Mir SS, Eiben LJ, Lewis J, Reffey SB, Frattini A, Tian L, Frank S, Youle RJ, Nelson DL, Notarangelo LD, Vezzoni P, Fearnhead HO, Duckett CS. Molecular cloning of ILP-2, a novel member of the inhibitor of apoptosis protein family. Mol Cell Biol. 2001;21(13):4292