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ICAD, CT, Blocking Peptide (Inhibitor of Caspase Activated Deoxyribonuclease)

Cat no: I0505P

ICAD, CT, Blocking Peptide (Inhibitor of Caspase Activated Deoxyribonuclease)

Peptide corresponding to amino acids 312 to 331 of mouse ICAD (2). \nApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis (1). Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase (2,3). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (2,3). Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death (4).

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SPECIFICATIONS

Catalog Number

I0505P

Size

50ug

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.

Purity

Purified 60-70%

References

1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184 \n\n2. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50 \n\n3. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99 \n\n4. Wyllie A. Apoptosis. An endonuclease at last. Nature 1998;39120-21\n

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