ICAD (Inhibitor of CAD, ICAD-L, ICAD-S, DNA Fragmentation Factor Subunit alpha, Dffa, DFF35, DNA Fragmentation Factor 45 kD Subunit, Dff45, DFF-45)

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A Human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis (1). mouse homologue of Human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase (2,3). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (2,3). Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death (4).\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500 - 1:1000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMouse lung\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

I0505-05C

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184. 2. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50. 3. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99. 4. Wyllie A. Apoptosis. An endonuclease at last. Nature 1998;391:20-21. 5. Muzio M, et al. Cell 85: 817-827 (1996). 6. Li P, et al. Cell 91: 479-489 (1990). 7. Cohen GM. Biochem. J. 326:1-16 (1997).

Additional Info

Recognizes mouse ICAD.

Alternative Names

DNA Fragmentation Factor, alpha Subunit, A330085O09Rik