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ICAD, Recombinant, Human (DNA Fragmentation Factor Subunit alpha, DNA Fragmentation Factor 45kD Subunit, DFF-45, Inhibitor of CAD, DFFA, DFF1, DFF45, H13)

ICAD, Recombinant, Human (DNA Fragmentation Factor Subunit alpha, DNA Fragmentation Factor 45kD Subunit, DFF-45, Inhibitor of CAD, DFFA, DFF1, DFF45, H13)

Cat no: I0505-05V

Supplier: United States Biological
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The inhibitor of caspase-3-activated DNase (ICAD) is a caspase 3 substrate that controls nuclear apoptosis. ICAD has two isoforms: a functional isoform of 45kD, ICAD-L/DNA fragmentation factor (DFF) 45; and a 35kD isoform, ICAD-S/DFF35. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD. In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone. There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells. Source: Recombinant corresponding to aa1-331 from human ICAD, fused to N-His tag expressed in E.coli. Molecular Weight: ~38kD Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: I0505-05V
Size: 200ug
Form: Supplied as a lyophilized powder from 50mM NaH2PO4, pH8.0, 300mM sodium chloride, 130mM imidazole.
Purity: ~95% (SDS-PAGE)
References: 1. Enari M et al., 1998, Nature 391(6662):43-50. 2. Sakahira H et al., 1999, J Biol Chem 274(22):15740-4. 3. Charrier L et al., 2002, Cancer Res 62(7):2169-74. 4. Fukushima K et al., 2002, J Mol Biol 321(2):317-27.

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