Iceberg

A novel protein, ICEBERG, an inhibitor of pro-caspase 1 activation, has been studied. It consists essentially only of a CARD, inhibits generation of IL-1b by interacting with caspase-1 and preventing association with RIP 2. The ICEBERG functions as a competitive antagonist of RIP 2/pro-caspase 1 interactions and inhibits caspase 1 dependent pro-L-1b secretion in mononuclear cells. ICEBERG is induced by proinflammatory stimuli, suggesting that it may be part of a negative feedback loop.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I0620-08

Size

50ug

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

PBS with 0.02% sodium azide

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Razmara M et al. CARD-8 protein, a new CARD family member that regulates caspase-1 activation and apoptosis. J Biol Chem 277:13952-8 (2002). <PUBMED:11821383>Druilhe A et al. Regulation of IL-1beta generation by Pseudo-ICE and ICEBERG, two dominant negative caspase recruitment domain proteins. Cell Death Differ 8:649-57 (2001). <PUBMED:11536016>Lee SH et al. Cop, a caspase recruitment domain-containing protein and inhibitor of caspase-1 activation processing. J Biol Chem 276:34495-500 (2001). <PUBMED:11432859>Humke EW et al. ICEBERG: a novel inhibitor of interleukin-1beta generation. Cell 103:99-111 (2000). <PUBMED:11051551>

Additional Info

This antibody reacts predominantly with ICEBERG. Species Crossreactivity: Crossreacts with Human and Rat.Not yet tested in other species.