ICOS (CD278, Inducible T cell co stimulator, Activation inducible lymphocyte immunomediatory molecule, AILIM, MGC39850)

The inducible costimulator (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Secondary signaling through CD28 or ICOS results in discrete cytokine secretion profiles by the activated T cells(1)/ Signaling by either molecule is effectively down regulated by CD152 (CTLA-4) engagement(2). Human GL50 is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be the ligand for ICOS.3 Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain.\n\nApplications: \nSuitable for use in ELISA and Flow Cytometry and Functional application. Other applications not tested.\n\nRecommended Dilution:\nFunctional Application: Antibody blocks binding of Recombinant GL50-muIg to ICOS\nFlow Cytometry: 1ug/ml. 5x10e5 human HPB-MLT cells were washed, incubated for 45 min. on ice with 80ul of \nI0650-01 at 1ug/ml. Cells were washed twice and incubated appropriate secondary antibody, after which they were washed twice, fixed and analyzed by FC. Cells stained positive with a mean shift of 1.3 log10 fluorescent units when compared to isotype control mouse IgG.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I0650-01

Size

100ug

Applications

ELISA, FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.5, 0.5 mg/ml Gentamicin Sulfate.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1,k

References

1) Beier, K.C., R.A. Kroczek, et al. 2000, Eur J Immunol. 30(12):3707-3717. \n2) Riley, J.L., C.H. June, et al. 2001, J. Immunol.166 : 4943-4948. \n3) Ling, V., M. Collins, et al. 2000, J. Immunol.164: 1653-1657. \n4) Ling, V., M. Collins, et al. 2001, J. Immunol.166: 7300-7308.

Additional Info

Binds to recombinant and cell surface ICOS.