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ICOS (CD278, Inducible T cell co stimulator, Activation inducible lymphocyte immunomediatory molecule, AILIM, MGC39850) (PE)

Cat no: I0650-01B

ICOS (CD278, Inducible T cell co stimulator, Activation inducible lymphocyte immunomediatory molecule, AILIM, MGC39850) (PE)

The inducible costimulator (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Secondary signaling through CD28 or ICOS results in discrete cytokine secretion profiles by the activated T cells.1 Signaling by either molecule is effectively down regulated by CD152 (CTLA-4) engagement.2 Human GL50 is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be the ligand for ICOS.3 Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain. 4\n\nFunctional Application: Antibody blocks binding of Recombinant GL50-muIg to ICOS\n\nApplications: \nSuitable for use in Flow Cytometry and ELISA. Other applications not tested.\n\nRecommended Dilution\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.

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SPECIFICATIONS

Catalog Number

I0650-01B

Size

120Tests

Applications

ELISA, FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1) Beier, K.C., R.A. Kroczek, et al. 2000, Eur J Immunol. 30(12):3707-3717. \n2) Riley, J.L., C.H. June, et al. 2001, J. Immunol.166 : 4943-4948. \n3) Ling, V., M. Collins, et al. 2000, J. Immunol.164: 1653-1657. \n4) Ling, V., M. Collins, et al. 2001, J. Immunol.166: 7300-7308.

Additional Info

Binds to recombinant and cell surface ICOS.

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