ICOS (Inducible co-stimulator, AILIM, CD278, CRP-1) (PE)

Inducible co-stimulator (ICOS), also known as AILIM (activiation- inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28- related protein-1), is a member of the CD28 family of immune co-stimulatory receptors. Other family members are CD28, CTLA-4, and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121aa extracellular domain, a 23 aa transmembrane region, and a 35aa cytoplasmic domain. ICOS shares ~39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share ~72% amino acid identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within ~24 - 48 hours of activation on T helper (Th) primed cells. B7-H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ ICOS interaction appears to play roles in T cell-dependent B cell activation and T helper cell differentiation.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 10ul, 1x10e6 cells\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nMouse myeloma cells with B cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

145489

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).

P Type

Mab

Purity

Purified by Protein A or G affinity chromatography.

Isotype

IgG1

References

1. Aicher, A. et al. (2000) J. Immunol. 164:4689. 2. Coyle, A.J. et al. (2000) Immunity 13:95. 3. Coyle, A.J. & J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203. 4. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1. 5. Hutloff, A. et al. (1999) Nature 397:263. 6. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040. 7. Yoshinaga, S.K. et al. (1999) Nature 402:827.

Additional Info

Recognizes human ICOS.