ICOS Ligand (CD275, Inducible T cell co-stimulator ligand, B7-H2, B7 homolog 2, B7-like protein Gl50, B7 related protein 1, B7H2, B7RP-1, B7RP1, GL50, ICOS LG , ICOSLG, KIAA0653, LICOS, Transmembrane protein B7 H2 ICOS ligand)

B7-H2 is detected on the surface of monocyte derived immature dendritic cells. It is a putative ligand for ICOS (inducible costimulator) from CD28 family of T cell-costimulators. Costimulatory interactions between the B7 family ligands and their receptors like CD28, CTLA-4 and ICOS play critical role in the growth, differentiation and death of T cells. B7-H2 has four conserved structural cysteins in its extracellular domain which are believed to form the Ig V like and IgC-like domain. In addition, B7-H2 has a conserved tyrosine residue in the Ig V-like domain at position of aa80. B7-H2 shares ~46% homology at amino acid level to B7h/B7RP-1, a recently described B7 like protein of mouse origin. \n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: Tested on BOSC cells transiently transfected with an ICOS Ligand expression vector.\nOptimal conditions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

I0650-04

Size

100ug

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

T1. Tumor necrosis factor-alpha regulates the expression of inducible costimulator receptor ligand on CD34(+) progenitor cells during differentiation into antigen presenting cells: G. Richter, et al.; J. Biol. Chem. 276, 45686 (2001)

Additional Info

Recognizes the extracellular domain of human ICOS Ligand native protein on antigen presenting cells.