ICOSL (B7-H2, CD275, ICOS Ligand, B7 Homolog 2, B7-like Protein Gl50, B7-related Protein 1, B7RP-1, ICOSLG, B7H2, B7RP1, KIAA0653)

B7-H2, or Inducible costimulator ligand (ICOSL), is a member of the Ig superfamily and belongs to the B7 family of costimulatory molecules. It specifically binds to ICOS, but not to other T cell costimulatory molecules such as CD28 and CTLA-4. B7-H2-ICOS interaction appears to be very important in the expansion of activated T cells and late-phase immune responses (1). B7-H2 enhances the proliferation of both CD4 and CD8 T cells, the secretion of Th1- and/or Th2-type cytokines, and the expression of CD154 (CD40 ligand) on T cells (2). It is expressed constitutively on B lymphocytes and at low levels on monocytes (3). Furthermore, B7-H2-ICOS interaction induces IL-10 production, which plays an important role in reducing immune responses (4).\n\nApplications:\nSuitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern Blot: 1:1000-1:10,000 \nImmunocytochemistry: 1:50-1:100\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

045629

Size

100ul

Applications

ICC, WB

Hosts

Rabbit

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

References

1. Kadkhoda K et al. J Immunol. 2010 Apr 1;184(7):3780-8. Epub 2010 Feb 26. 2. Maeda M et al. MAbs. 2009 Sep-Oct;1(5):453-61. Epub 2009 Sep 26. 3. Wong SC et al. Blood. 2003 Aug 15;102(4):1381-8. Epub 2003 Apr 24. 4. Yamashita T et al. Clin Cancer Res. 2009 Feb 1;15(3):770-7.

Additional Info

Recognizes human ICOSL.