IDO (Indoleamine 2,3-dioxygenase, INDO)

IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T-cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T-cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.\n\nApplications:\nSuitable for use in ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot\n\nRecommended Dilution:\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

044893

Size

100ug

Applications

ELISA, ICC, IHC, WB

Hosts

Rabbit

Form

Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

References

Product Reference: \n?Blockade of Indoleamine 2,3-Dioxygenase Protects Mice against Lipopolysaccharide-Induced Endotoxin Shock: I.D. Jung, et al.; J. Immunol. 182, 3146 (2009) \n?A combination of the metabolic enzyme inhibitor APO866 and the immune adjuvant L-1-methyl tryptophan induces additive antitumor activity: H.J. Yang, et al.; Exp. Biol. Med. (Maywood) 235, 869 (2010)\n?

Additional Info

Recognizes mouse IDO. Detects a band of ~45kD by Western blot.

Alternative Names

Indoleamine 2,3-dioxygenase, INDO