IER3 (Radiation-inducible Immediate-early Gene IEX-1, Differentiation-dependent Gene 2 Protein, Protein DIF-2, Immediate Early Protein GLY96, Immediate Early Response 3 Protein, PACAP-responsive Gene 1 Protein, Protein PRG1, DIF2, IEX1, PRG1)

IEX-1 is a stress inducible gene that is induced by ionizing radiation, ultraviolet radiation, and a variety of growth factors, i.e., FAS and TNF-a. IEX-1 is widely expressed in epithelial and endocrine tissues, as well as in vascular endothelium. It plays an important role in the regulation of cellular growth, cell death and oncogenesis. IEX-1 is precisely regulated by multiple transcription factors such as p53, NF-kappaB/rel, Sp1 and c-Myc, to ensure rapid and transient expression of IEX-1 in cells under a variety of stress conditions. IEX-1 is expressed as both a longer form (IEX1L) and a splice variant, designated IEX1S. It is localized to the nucleus and perinuclear region. Overexpression of IEX-1 facilitates apoptosis and cell cycle progression, whereas disruption of IEX-1 expression is associated with decreases in both apoptosis and cell cycle progression.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 2-4ug/ml\nImmunofluorescence: 20ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman Brain Tissue Lysate or Human Liver Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146172

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Kondratyev AD, Chung K-N and Jung MO. Identification and characterization of a radiation-inducible glycosylated human early-response gene. Cancer Res. 1996; 56:1498-502. 2. Feldmann KA, Piddelkow MR, Roche PC, et al. Expression of an immediate early gene, IEX-1, in human tissues. Histochem. Cell Biol. 2001; 115:48997. 3. Wu MX, Zhaohui A, Prasad KVS, et al. IEX-1L, an apoptosis inhibitor involved in NFkappaB mediated cell survival. Science 1998; 281:998-1001. 4. Arlt A, Grobe O, Sieke A, et al. Expression of the NF-kappa B target gene IEX-1 (p22/PRG1) does not prevent cell death but instead triggers apoptosis in Hela cells. Oncogene 2001; 20:6976.

Additional Info

Recognizes human IEX1. Species Crossreactivity: mouse.