IFN-gamma, Porcine, BioAssay(TM) DuoSet ELISA Development Kit (IFNG)

Interferon-gamma (IFN-gamma, also known as Type II interferon or immune interferon) is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-beta or the various IFN-alpha family proteins. Mature IFN-g exists as noncovalently-linked homodimers. Human IFN-gamma is highly species specific and is biologically active only in human and primate cells. IFN-gamma was originally characterized based on its antiviral activities. The protein also exerts anti-proliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-gamma induces the production of cytokines, up-regulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-gamma also augments Helper T cell expansion and may be required for Helper T cell differentiation. IFN-gamma exerts its biological activities by binding to specific cell surface receptors with high-affinity binding sites. The IFN-gamma receptor is present on almost all cell types except mature erythrocytes and has been cloned and characterized. The IFN-gamma receptor is structurally related to the IL-10 receptor.\n\nSample Type:\nCell culture supernates\n\nIntended Use:\nThis BioAssay(TM) DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant porcine Interferon gamma (IFN-g). DuoSets are designed for the analysis of cell culture supernates. \n\nRange:\n~62.5-4000pg/ml\n\nSpecificity:\nRecognizes natural and recombinant porcine Interferon gamma (IFN-g). \n\nKit Components:\nMouse anti-porcine IFN-g capture antibody, 1x vial\nBiotinylated goat anti-porcine IFN-g detection antibody, 1x vial\nRecombinant porcine IFN-g standard, 1x 3vials\nStreptavidin-HRP, 1x vial\n\nStorage and Stability:\nSttore other components at 4 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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