IgA, Secretory Component

IgA is the predominant immunoglobulin class in body secretions, such as saliva, tears, bronchial secretions, nasal mucosal secretions, prostatic fluid, vaginal secretions, and mucous secretions of the small intestines. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. It is also found in small amounts in blood. Because it is resistant to degradation by enzymes, secretory IgA can survive in harsh environments such as the digestive and respiratory tracts, to provide protection against microbes that multiply in body secretions. IgA does not activate complement, and opsonises only weakly. Its heavy chains are of the type . It exists in two forms, IgA1 (90%) and IgA2 (10%): IgA1 is found in serum and made by bone marrow B cells. In IgA2, the heavy and light chains are not linked with disulfide but with noncovalent bonds. IgA2 is made by B cells located in the mucosae and has been found to secrete into colostrum, maternal milk, tears and saliva.\n\nApplications:\nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilutions:\nDirect ELISA: 1:20,000 dilution will yield >1.0 O.D. using alkaline phosphatase conjugated rabbit anti-mouse Ig.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

I1890-33

Size

500ug

Applications

ELISA

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 500ul sterile ddH2O.

P Type

Mab

Purity

Purified by Ion Exchange chromatography.

Isotype

IgG1

References

1. Secretory IgA in saliva and academic stress. Otsuki T, Sakaguchi H, Takata A, Morimoto K, Int J Immunopathol Pharmacol 2004 May-Aug;17(2 Suppl):45-8 2. Secretory IgA antibodies provide cross-protection against infection with different strains of influenza B virus. Asahi-Ozaki Y, Yoshikawa T, Suzuki Y, J Med Virol 2004 Oct;74(2):328-35 3. Antigen-specific secretory IgA antibodies in the gut are decreased in a mouse model of food allergy. Frossard CP, Hauser C, J Allergy Clin Immunol 2004 Aug;114(2):377-82 4. Evaluation of seven assays detecting serum immunoglobulin classes and subclasses and salivary and faecal secretory IgA against Fasciola excretory/secretory (ES) antigens in diagnosing fascioliasis. Noureldin MS, el-Ganaini GA, el-Nemr HE, J Egypt Soc Parasitol 2004 Aug;34(2):691-704 5. Genotyping of human giardiasis in relation to anti-Giardia secretory IgA and mucosal histopathology. Risk H, el-Shazly AM, Mohamad AA, J Egypt Soc Parasitol 2004 Aug;34(2):471-81 6. Dual roles of PspC, a surface protein of Streptococcus pneumoniae, in binding human secretory IgA and factor H. Dave S, Carmicle S, Pangburn MK, J Immunol 2004 Jul 1;173(1):471-7.

Additional Info

Recognizes all human IgA subtypes.