IGF-1 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human IGF-1 in cell culture supernates, serum and plasma (heparin, EDTA).\n\nInsulin-like growth factor 1 (IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6kD.1 The IGF1 gene, mapped on 12q22-q24.1, contains 5 exons. Exons 1-4 encode the 195-amino acid precursor (IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide (IGF1A).2 The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation.3 IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.\n\nAssay Range:\n62.5pg/ml-4000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with IGF-2.\n\nUniprot ID:\nP05019\n\nEntrez Gene ID:\n3479\n\nKit Components:\n96-well plate pre-coated with anti-IGF-1, Human\nLyophilized recombinant IGF-1, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143870

Size

96Tests

References

1. Rinderknecht, E.; Humbel, R. E.: The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin. J. Biol. Chem. 253: 2769-2776, 1978. \n2. Rotwein, P.; Pollock, K. M.; Didier, D. K.; Krivi, G. G.: Organization and sequence of the human insulin-like growth factor I gene: alternative RNA processing produces two insulin-like growth factor I precursor peptides. J. Biol. Chem. 261: 4828-4832, 1986.\n3. Sussenbach, J. S.; Steenbergh, P. H.; Holthuizen, P.: Structure and expression of the human insulin-like growth factor genes. Growth Regul. 2: 1-9, 1992.\n