IGF-2 BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse IGF-2 in cell culture supernates, serum and plasma (heparin, EDTA).\n\nInsulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth.1 It is paternally expressed in the fetus and placenta.2 IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues.3 IGF-II appears to be induced by placental lactogen during prenatal development.4 It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.5 \n\nAssay Range:\n62.5pg/ml-4000pg/ml\n\nSensitivity:\n< 5 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with IGF-1.\n\nUniprot ID:\nP09535\n\nEntrez Gene ID:\n16002\n\nKit Components:\n96-well plate pre-coated with anti-IGF-2, Mouse\nLyophilized recombinant IGF-2, Mouse standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143874

Size

96Tests

References

1. Bachner-Melman, R.; Zohar, A. H.; Nemanov, L.; Heresco-Levy, U.; Gritsenko, I.; Ebstein, R. P. Association between the insulin-like growth factor 2 gene (IGF2) and scores on the Eating Attitudes Test in nonclinical subjects: a family-based study. Am. J. Psychiat. 162: 2256-2262, 2005. \n2. Constancia, M.; Hemberger, M.; Hughes, J.; Dean, W.; Ferguson-Smith, A.; Fundele, R.; Stewart, F.; Kelsey, G.; Fowden, A.; Sibley, C.; Reik, W. Placental-specific IGF-II is a major modulator of placental and fetal growth. Nature 417: 945-948, 2002. \n3. Ogawa, O.; Becroft, D. M.; Morison, I. M.; Eccles, M. R.; Skeen, J. E.; Mauger, D. C.; Reeve, A. E. Constitutional relaxation of insulin-like growth factor II gene imprinting associated with Wilms' tumour and gigantism. Nature Genet. 5: 408-412, 1993. \n4. Dull, T. J.; Gray, A.; Hayflick, J. S.; Ullrich, A. Insulin-like growth factor II precursor gene organization in relation to insulin gene family. Nature 310: 777-781, 1984. \n5. Brisken, C.; Ayyannan, A.; Nguyen, C.; Heineman, A.; Reinhardt, F.; Tan, J.; Dey, S. K.; Dotto, G. P.; Weinberg, R. A. IGF-2 is a mediator of prolactin-induced morphogenesis in the breast. Dev. Cell 3: 877-887, 2002. Note: Erratum: Dev. Cell 4: 283 only, 2003. \n