Human IGF-BP1 is a 25.4kD cysteine-rich secreted protein expressed in liver, decidual, and kidneys and is the most abundant IGF-BP in amniotic fluid. Levels of IGF-BP1 in serum are lowest after food. IGF-BP1 binds both IGF-I and IGF-II with equal affinity. Phosphorylated IGF-BP1 hinders IGF actions, whereas nonphosphorylated IGF-BP1 is stimulatory. Recombinant human IGF-BP1 is a single, glycosylated protein consisting of 255aa residues with a N-terminal His-tag.
Source:
Recombinant corresponding to human IGF-BP1 expressed in Nso cells.
Molecular Weight:
~28kD
Biological Activity:
The ED50 as determined by its ability to inhibit IGF-I induced proliferation of NUC-1 cells is ~100ng/ml.
Endotoxin:
~0.1ng/ul
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.