IGF1, Human, BioAssay(TM) Kit (Insulin-like Growth Factor I, IGF-I, Mechano Growth Factor, MGF, Somatomedin-C, IBP1)

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I is synthesized as two precursor isoforms with N- and alternate C-terminal pro-peptides. These isoforms are differentially expressed by various tissues. The 7.6kD mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature human IGF-I shares 94% and 96% aa sequence identity with mouse and rat IGF-I, respectively, and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF-II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects.1 IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally. IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism. It plays an important role in muscle regeneration and tumor progression. IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R.9 IGF-I association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors.\n\nIntended Use:\nDesigned to qualitatively and quantitatively determine the presence of cells expressing IGF-I receptors or other interacting proteins by flow cytometry.\n\nSpecificity:\nHuman\n\nTest Principle:\nWashed cells are incubated with the biotinylated protein that in turn binds to the cells via specific cell surface receptors or other interacting proteins. The cells are then directly incubated with avidin-fluorescein, which attaches to the bound biotinylated protein. Cells expressing the receptor/interacting protein are fluorescently stained, with the intensity of staining proportional to the density of such protein. Relative density is then determined by flow cytometry using 488nm wavelength laser excitation.\n\nKit Components:\nRecombinant human IGF-I conjugated to Biotin: 1x100 reactions\nAvidin-Fluorescein: 1x2ml\nNegative Control Reagent: 1x600ul\nBlocking Antibody: 1x15 reactions\nCell Wash Buffer (10 X): 1x60ml\n\nStorage and Stability:\nSee Kit Protocol for detailed storage instructions.

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SPECIFICATIONS

Catalog Number

I7661-11P

Size

1Kit

References

1. Philippou, A. et al. (2007) In Vivo 21:45. 2. Sandberg-Nordqvist, A.C. et al. (1992) Brain Res. Mol. Brain Res. 12:275. 3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890. 4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620. 5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577. 6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275. 7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10. 8. Pollak, M. (2008) Nat. Rev. Cancer. 8:915. 9. LeRoith, D. & S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302. 10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421. 11. Chakraborty et al. (2008) Cancer Res. 68:1538.