IGFBP-1 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human IGFBP-1 in cell culture supernates, serum, plasma (heparin, EDTA) and urine.\n\nIGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25.1 The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth.2 These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II.3 The gene is organized in four exons and spans 5.9 kb.4 The IBP-1 gene is a single copy gene, located on chromosome 7.5 The standard product used in this kit is recombinant human IGFBP-1, consisting of 234 amino acids with the molecular mass of 25kD.\n\nAssay Range:\n31.2pg/ml-2000pg/ml\n\nSensitivity:\n< 1 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP08833\n\nEntrez Gene ID:\n3484\n\nKit Components:\n96-well plate pre-coated with anti-IGFBP-1, Human\nLyophilized recombinant IGFBP-1, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143876

Size

96Tests

References

1. Ballard, F. J.; Baxter, R. C.; Binoux, M.; Clemmons, D. R.; Drop, S. L. S.; Hall, K.; Hintz, R. L.; Rechler, M. M.; Rutanen, E. M.; Schwander, J. C. Report on the nomenclature of the IGF binding proteins. J. Clin. Endocr. Metab. 70: 817-818, 1990. \n2. Alitalo, T.; Kontula, K.; Koistinen, R.; Aalto-Setala, K.; Julkunen, M.; Janne, O. A.; Seppala, M.; de la Chapelle, A. The gene encoding human low-molecular weight insulin-like growth-factor binding protein (IGF-BP25): regional localization to 7p12-p13 and description of a DNA polymorphism. Hum. Genet. 83: 335-338, 1989. \n3. Ekstrand, J.; Ehrenborg, E.; Stern, I.; Stellan, B.; Zech, L.; Luthman, H. The gene for insulin-like growth factor binding protein-1 is localized to human chromosomal region 7p14-p12. Genomics 6: 413-418, 1990. \n4. Brinkman, A.; Groffen, C. A. H.; Kortleve, D. J.; Drop, S. L. S. Organization of the gene encoding the insulin-like growth factor binding protein IBP-1. Biochem. Biophys. Res. Commun. 157: 898-907, 1988.\n5. Brinkman, A.; Groffen, C.; Kortleve, D. J.; Geurts van Kessel, A.; Drop, S. L. S. Isolation and characterization of a cDNA encoding the low molecular weight insulin-like growth factor binding protein (IBP-1). EMBO J. 7: 2417-2423, 1988.\n