IGFBP-2, Human BioAssay(TM) ELISA Kit (Insulin Like Growth Factor Binding Protein 2)

The superfamily of secreted insulin-like growth factor (IGF) binding proteins includes high-affinity IGF binding proteins (IGFBP) and low-affinity binding proteins referred to as IGFBP-like or IGFBP- related proteins (IGFBPL, IGFBP-rP) (1). IGFBPs form complexes with IGF-I and -II, modulating their activity and bioavailability by modifying their binding to cell receptors, cell surfaces, and the extracellular matrix (1-5). In addition to direct effects on activity and binding of IGFs, IGFBPs act\nas carriers for circulating IGFs, prolonging their half-lives, and creating IGF reservoirs in tissues (1, 3, 4). IGFBP-2 and other superfamily members contain N-terminal IGF-binding domains with 12 conserved cysteines, and C-terminal thyroglobulin type 1 domains with 6 conserved cysteines (1). The C-terminal domain mediates heparin binding, which is enhanced in the presence of IGFs and, in the case of IGFBP-2, contains an RGD sequence that promotes integrin-mediated cell attachment (1, 3, 5-9). IGFBP-2 affinity and extracellular matrix binding enhancement is stronger for IGF-II than IGF-I (4, 9).\n\nIntended Use:\nFor the quantitative determination of Insulin-like Growth Factor Binding Protein 2 (IGFBP-2) concentrations in cell culture supernates, serum, plasma, and urine.\n\nSensitivity:\n0.09ng/ml\n\nRange:\n~0.313-20ng/ml\n\nTest Principle:\nThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IGFBP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGFBP-2 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for IGFBP-2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGFBP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.\n\nKit Components:\nMicroplate, 1x96wells\nConjugate (HRP), 1x21ml\nStandard, 1x200ng\nAssay Diluent, 1x11ml\nCalibrator Diluent, 1x2vials\nWash Buffer Concentrate, 1x21ml\nColor Reagent A, 1x12ml\nColor Reagent B, 1x12ml \nStop Solution, 1x6ml\nPlate Sealers, 1x4 4 adhesive strips.\n\nStorage and Stability:\nStore other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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