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IgG, F(ab

Cat no: I1904-19A

IgG, F(ab

DTAF has absorption and emission properties nearly identical to fluorescein isothiocyanate (FITC) and DTAF- and FITC-labeled antibodies are similar in terms of preparation and specificity of immunofluorescence. However, DTAF is superior to FITC with regard to cost, purity and stability. Also, DTAF-labeled rabbit IgG conjugates can be quickly and efficiently fractionated by simple ammonium sulfate precipitation to yield preparations free of both over- and under-conjugated material. In addition, over-conjugated protein can be readily removed from DTAF:IgG conjugates by appropriate adjustment of pH and temperature.\n\nApplications: \nSuitable for use in FLISA, Immunofluorescence and Fluorescent Western Blot. Other applications not tested. \n\nRecommended Dilutions: \nFLISA: 1:10,000-1:50,000\nImmunofluorescence: 1:1000-1:5000\nOptimal dilutions to be determined by the researcher.\n\nLabel (FITC):\nAbsorption Wavelength: 495nm\nEmission Wavelength: 528nm\nFluorochrome/Protein Ratio: 3 moles FITC per mole of IgG\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

I1904-19A

Size

1mg

Applications

ELISA, IF, WB

Hosts

Goat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with FITC.

P Type

Pab

Purity

Purified by immunoaffinity chromatography

Isotype

IgG

Additional Info

Recognizes mouse IgG, F(ab

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