IgG, Fc (FITC)

Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons)\n\nAbsorption Wavelength: 495 nm\n\nEmission Wavelength: 528 nm\n\nFluorochrome/Protein Ratio: 2.3 moles FITC per mole of Rabbit IgG\n\nApplication(s): Suitable for use in ELISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications have not been tested. \n\nRecommended Dilution(s): Not specified. Optimal titers for applications should be determined by the researcher.\n\nStorage Conditions: Store vial at 4 degrees C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at-20 degrees C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

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SPECIFICATIONS

Catalog Number

I1904-59L

Size

500ug

Applications

ELISA, IF, WB

Hosts

Rabbit

Reactivities

Sheep

Form

FITC conjugated. Lyophilized in 0.02 M Potassium Phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA (IgG and Protease free).\\ 0.01% sodium azide

P Type

Pab

Purity

Prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads.

Isotype

IgG

Additional Info

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum. No reaction was observed against Sheep IgG F(ab