IgG (H&L) (PE), X-Adsorbed

Applications: \nSuitable for Immunomicroscopy, Immunofluorescence, Flow Cytometry and FACS Analysis as well as other antibody based ?uorescent assays. Other applications not tested.\n\nRecommended Dilution: \nFlow Cytometry: 1:100-1:200 (~1ug PE conjugate will stain 1x10e6 cells).\nIF Microscopy: 1:100-1:200\nOptimal dilutions to be determined by the researcher.\n\nLabel: \nR-Phycoerythrin (PE), 0.5mg/ml (determined by Abs: 82.0 at 565nm for a 1% solution for only those molecules to which at least one molecule of active antibody is bound).\n\nMW of Label: \n240kD\n\nAbsorption Wavelength: \n490, 545, 565nm\n\nEmission Wavelength:\n580nm\n\nOD566nm/OD280nm:\n2.6\n\nNote: \nConcentration value is Phycobiliprotein concentration. It is determined by absorbance = 82.0 at 565nm for a 1% solution for only those R-PE molecules to which one molecule of active antibody is bound.\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light and cold.

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SPECIFICATIONS

Catalog Number

I1903-13B

Size

500ul

Applications

FC, IF

Reactivities

Rabbit

Form

Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Labeled with R-Phycoerythrin (PE).

P Type

Pab

Purity

Purified by immunoaf?nity chromatography.

Isotype

F(ab

Additional Info

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins.