IgG, H&L, X-Adsorbed (HRP)

Applications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:10,000-1:50,000\nWestern Blot: 1:10,000-1:50,000\nImmunohistochemistry: 1:500-1:5000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

I1903-89U6

Size

500ug

Applications

ELISA, IHC, WB

Hosts

Goat

Reactivities

Rabbit

Form

Supplied as a lyophilized powder in 10mM sodium phosphate, 0.15M sodium chloride, pH7.2, 1% BSA, Protease/IgG free, 0.1% Kathon CG. Labeled with horseradish peroxidase (HRP).

P Type

Pab

Purity

~90% based on SDS-PAGE, may contain small amounts of intact IgG.

Isotype

F(ab

Additional Info

Recognizes heavy chains on rabbit IgG light chains on all rabbit immunoglobulins based on IEP. Does not recognize non-immunoglobulin rabbit serum proteins. Does not recognize serum proteins from bovine, human, or mouse or IgG from human or mouse.