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IgG. Rabbit Isotype Control (Alexa Fluor(R) 647)

Cat no: I1903-93

IgG. Rabbit Isotype Control (Alexa Fluor(R) 647)

Isotype control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type as the test antibody. Rabbit IgG was conjugated to Alexa Fluor(R) 647 fluorescent dye under optimal conditions, and tested in-house for direct flow cytometric analysis of human and mouse cells. Alexa Fluor(R) dye is maximally excited by red light (e.g. 633nm He-Ne laser). Antibody conjugated of the Alexa Fluor(R) 647 dye produce far-red-fluorescence emission with a peak at 655nm.\n\nDirections on Use:\nThis control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. Higher background fluorescence may result if excessive amounts of rabbit IgG isotype control are used. Do not use this antibody at 1:10 dilution in the same way as recommended for other Alexa Fluor(R) conjugated antibodies.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4 degrees C. Freezing Alexa Fluor(R) conjugates will result in a substantial loss of activity. Alexa Fluor(R) conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

I1903-93

Size

100ul

Applications

FC

Form

Supplied as a liquid in PBS, pH 7.2, 2mg/ml BSA, <0.1% sodium azide. Labeled with Alexa Fluor(R) 647 (F/P ratio of 2-5)

Purity

Purified by affinity chromotography

Isotype

IgG

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