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IgG1 Murine Negative Control (For rat tissue) (Azide Free)

Cat no: I1904-78Z

IgG1 Murine Negative Control (For rat tissue) (Azide Free)

Applications:\nSuitable for use in Flow Cytometry used as a matched isotype negative control reagent to asses the level of non-specific binding of mouse IgG1 monoclonal antibodies to rat cells. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 10ul of the optimal working dilution to label 10e6 cells in 100ul.. Recommend adjusting the concentration of this reagent to the same as that of the test reagent.\nOptimal working dilutions to be determined by researcher.\n\nRecommended Positive Control: Human leukocytes\nRecommended Negative Control: Negative on the following rat tissues: peripheral blood leukocytes, thymocytes, splenocytes and macrophages.\n\nRecommended Secondary Reagents:\nGoat anti-mouse IgG labeled with FITC (rat adsorbed)\nGoat anti-mouse IgG labeled with PE (rat adsorbed)\n\nHybridoma:\nSpleen cells from immunized BALB/c mice were fused with cells of the NSI mouse myeloma cell line.\n\nSource: Tissue culture supernatant\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I1904-78Z

Size

1mg

Applications

FC

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS. No preservatives added.

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human CD45RA antigen. Note: Use with rat tissues only.

Read more on Supplier website

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info
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