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IgG1 Murine Negative Control (PE)

Cat no: I1904-79D

IgG1 Murine Negative Control (PE)

Negative on all human cells and cell lines tested. Also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea pig tissues. Recognizes a rat cell surface marker. Cannot be used as a negative control in rat.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution: \nFlow Cytometry: Neat; 10ul labels 10e6 separated cells or 100ul lysed whole peripheral blood.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C. Reconstitute with sterile ddH2O. Aliquot and store at 4 degrees C. DO NOT FREEZE. Reconstituted product is stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

I1904-79D

Size

100Tests

Applications

FC

Reactivities

Mouse

Form

Supplied as a lyophilized powder from PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with PE. Reconstitute with 1ml sterile dH2O.

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Malhotra, R., et al., Biochem. J. 320: 589-593 (1996). 2. Kupatt, C., et al., Arterioscter. Thromb. Vasc. Biol. 20(10): 2226-2232 (2000). 3. Dalli, J., et al., Blood 112: 2512-2519 (2008). 4. Barratt-Due, A., et al., J. Immunol. 187: 4913-4919 (2011).

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