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IgG1 Negative Control (PE)

Cat no: I1904-78P

IgG1 Negative Control (PE)

Applications: \nSuitable for use as a negative control in Immunofluorescence and Flow Cytometry. Its use enables an estimation of non-specific binding of fluorescein conjugated mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 10ul per 100ul whole blood, or 1x10e6 peripheral blood mononulear cells or bone marrow cells in 100ul buffer.\nImmunofluorescence: 10ul per 100ul whole blood\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.

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SPECIFICATIONS

Catalog Number

I1904-78P

Size

1ml

Applications

FC, IF

Hosts

Mouse

Reactivities

Ch/Bird

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. Conjugated to Phycoerythrin (PE).

Purity

Purified

Isotype

IgG1

Additional Info

Recognizes chicken immunoglobulin. No reaction with human cell surface or plasma components has been observed. However some binding to Fc receptors may occur.

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