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IgG1 Negative Control Rat (FITC)

Cat no: I1904-76L1

IgG1 Negative Control Rat (FITC)

Applications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: Neat. Use 10ul of the optimal dilution to label 10e6 cells in 100ul.\nOptimal dilutions to be determined by researcher.\n\nRecommended Secondary Reagents:\nStreptavidin labeled with RPE\nStreptavidin labeled with FITC\n\nFusion Partners: Spleen cells from immunized Lou/c rat were fused with cells of the rat IR983F myeloma cell line.\n\nLabel: Fluorescein isothiocyanate (FITC)\nMolecular Weight: 390D\nAbsorption Wavelength: 495nm\nEmission Wavelength: 528nm\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I1904-76L1

Size

100Tests

Applications

FC

Reactivities

Mouse

Form

Supplied as a liquid, 0.5% BSA, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

Purity

Purified by Protein A affinity chromatography.

Additional Info

Recognizes Dinitrophenyl Hapten. For use on mouse tissues or cells as a rat IgG1 isotype negative control for the measurement of non-specific binding of rat monoclonal antibodies of isotype IgG1.

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