IgG2

IgG is a glycoprotein that is produced by plasma cells in response to an immunogen (1). It is the only class of immunoglobulins that can cross the placenta, which is mediated by a receptor on placental cells in the Fc region (1). Human IgG2 subclass has been identified to exist as an ensemble of distinct isoforms, designated IgG2-A, -B, and -A/B, which differ by the disulfide connectivity at the hinge region (2). IgG2-A is the known classic structure for the IgG2 subclass defined by structurally independent Fab domains and hinge region (3). IgG2-B is a structure defined by a symmetrical arrangement of a (C(H)1-C(L)-hinge)(2) complex with both Fab regions covalently linked to the hinge (3). IgG2-A/B represents an intermediate form, defined by an asymmetrical arrangement involving one Fab arm covalently linked to the hinge through disulfide bonds (3).\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10,000-1:20,000\nImmunohistochemistry: 1:500-1:1000\nImmunocytochemistry: 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

I1904-80L

Size

100ul

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.

P Type

Mab

Purity

Supernatant

References

1. G Mayer. Immunoglobulin - Structure and Function. University of South Carolina School of Medicine. 2000 2. Dillon TM, et al. J Biol Chem 283(23):16206-15, 2008 3. Wypych J, et al. J Biol Chem 283(23):16194-205, 2008

Additional Info

Recognizes human IgG2 at ~52kD.