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IgG2a Negative Control

Cat no: I1904-82D

IgG2a Negative Control

Use of this negative control enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. \n\nApplications:\nSuitable for use as a negative control in Immunofluorescence, Flow Cytometry and Immunoperoxidase. Other applications have not been tested.\n\nRecommended Dilution:\nImmunoperoxidase: Staining frozen and paraffin sections.\nDilute to the same concentration as the test antibody, and equivalent volumes used. \n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nWarning: \nThe monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

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SPECIFICATIONS

Catalog Number

I1904-82D

Size

500ul

Applications

FC, IF

Hosts

Mouse

Form

Supplied as a liquid in 25mM Tris-HCl, 0.4M sodium chloride, pH 8.0, 0.2% BSA, 0.1% sodium azide, before the addition of glycerol to 40%.

Purity

Purified ascites. The characteristics of each lot are tested by electrophoresis, specific immunofluorescence assay and flow cytometry.

Isotype

IgG2a

Additional Info

Recognizes the outer membrane protein of some strains of Neisseria gonorrhoeae. No reaction with human cell surface or plasma components has been observed. However, some binding to Fc receptors may occur.

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