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IgG2b, Rat Negative Control

Cat no: I1904-82V3

IgG2b, Rat Negative Control

Hybridoma: \nSpleen cells from immunized LOU/c rats were fused with cells of the rat IR983F myeloma cell line.\n\nApplications:\nSuitable for use in Flow Cytometry. Also suitable for use as a negative control on human, mouse, bovine and canine tissues where rat monoclonals of isotype IgG2b are used. Other applications have not been tested.\n\nRecommended Dilutions:\nFlow Cytometry: 10ul labels 1x10e6 cells in 100ul.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I1904-82V3

Size

100ug

Applications

FC

Form

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide

Purity

Purified by affinity chromatography.

Isotype

IgG2b

References

1. Bellingan. G. et al. (2002) Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation. J. Exp. Med. 196:1515-1521.

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