IkB alpha, Cleaved

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). Recent studies have shown that IkBa can be cleaved by caspases during apoptosis to produce an amino-terminal truncated IkBa (DN-IkBa) (7,8). The DN-IkBa is resistant to degradation in response to inducers of NF-kB and act as dominant inhibitory molecule that suppresses NF-kB activity during apoptosis. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested. \n\nRecommended Dilution: \nWestern Blot: 1ug/ml\nOptimal dilution determined by the researcher. \n\nPositive Control:\nanti-Fas treated jurkat (30 min)\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

I2500-01H

Size

100ug

Applications

WB

Hosts

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Mab

Purity

Purified by Protein G Affinity Chromatography.

Isotype

IgG1

References

1. Verma, I.M., Stevenson, J.K., Schwarz, E.M., Van Antwerp, D. & Miyamoto, S. Genes. Dev. 9: 2723-2735, 1995. \n2. Verma, I. And Stevenson, J.K. Proc. Nat. Acad. Sci USA 94: 11758 (1997). \n3. DiDonato, J.A., Hayakawa, M., Rothwarf, D.M. Zandi, E., and Karin, M. Nature 388: 548- (1997). \n4. Regnier, C.H., Ho, Y.S., Gao, X., Goeddel, D.V., Cao, Z., and Rothe, M. Cell 90, 373 (1997). \n5. Mercurio, F., Zhu, H., Murray, B.W., Shevchenko, A., Bennett, B.L., Li, J., Young, D.B., Barbosa, M. Mann, M., Manning, A., Rao, A. Science 278: 860 (1997). \n6. Yamaoka, S., Courtois, G., Bessia, C., Whiteside, S.T., Weil, R., Agou, F., Kirk, H.E., Kay, R.J., and Israel, A. Cell 93: 1231-1240 (1998). \n7. Barkett, M, Xue, D, Horvitz, H.R., and Gilmore, TD. J Biol Chem 272: 29419-29422 (1997). \n8. Reuther, J.Y. and Baldwin, Jr., A.S. J Biol Chem 274: 20664-20670 (1999).

Additional Info

Recognizes human IkB alpha, Cleaved