IkB alpha

Supplier:

United States Biological

Cat no: I2500-05

IkB alpha

NFkB exists in the cytoplasm as a complex with a 38kD inhibitor protein, IkB (MAD-3). Activation of NFkB by different stimuli, including TNFa and PMA, requires the dissociation of the inhibitor protein. Dissociation is regulated by the phosphorylation of IkB on serine residues 32 and 36 which targets IkB for ubiquinitation and proteasome mediated degradation. The NFkB complex then translocates to the nucleus and activates transcription of responsive genes.\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml detects IkBa in RIPA lysates from A431 cells. A431cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with I2500-05 (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.\nImmunoprecipitation: 4ug immunoprecipitates IkBa from 500ug of A431 RIPA lysate.\nOptimal dilutions to be determined by the researcher.\n\nPositive Antigen Control Included: \nA0001-50: Non-stimulated A431 lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

I2500-05

Size

200ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Brown, et al., PNAS USA 90: 2532-2536 (1993).

Additional Info

Recognizes human IkBa (38kD). Species Crossreactivity: Weakly crossreacts with mouse.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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