IkB-Ras1 (IkB-interacting Ras-like Protein1)

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first genes induced following NF-kB activation is IkBa. Recently, two proteins, kB-Ras1 and kB-Ras2 have been identified (7). These two proteins interact with PEST domains of IkBa and IkBb and decrease their rate of degradation. \n\nApplication \nThis antibody can be used for detection of kB-Ras1 by Western blot analysis. \n\nRecommended Dilution:\nWestern blot: 0.5-1.0ug/ml \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I2503

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Verma, I.M., Stevenson, J.K., Schwarz, E.M., Van Antwerp, D. & Miyamoto, S. Genes. Dev. 9: 2723-2735, 1995. \n2. Verma, I. And Stevenson, J.K. Proc. Nat. Acad. Sci USA 94: 11758 (1997). \n3. DiDonato, J.A., Hayakawa, M., Rothwarf, D.M. Zandi, E., and Karin, M. Nature 388: 548- (1997). \n4. Regnier, C.H., Ho, Y.S., Gao, X., Goeddel, D.V., Cao, Z., and Rothe, M. Cell 90, 373 (1997). \n5. Mercurio, F., Zhu, H., Murray, B.W., Shevchenko, A., Bennett, B.L., Li, J., Young, D.B., Barbosa, M. Mann, M., Manning, A., Rao, A. Science 278: 860 (1997). \n6. Yamaoka, S., Courtois, G., Bessia, C., Whiteside, S.T., Weil, R., Agou, F., Kirk, H.E., Kay, R.J., and Israel, A. Cell 93: 1231-1240 (1998). \n7. Fenwick C, Na S-Y, Voll RE, Zhong H, Im S-Y, Lee JW, and Ghosh S. Science 287 (5454) 869-873 (2000).

Additional Info

Recognizes human IkB-Ras1 (IkB-interacting Ras-like Protein1)