IKBKE

Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon.The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degra-dation of IkB. IkB kinase a(IKKa), previously designated CHUK, interacts with IkB-aand specifically phosphorylates IkB-aon the sites that trigger its degradation, serines 32 and 36. The functional IKK complex contains three subunits, IKKa, IKKband IKKg(also designated NEMO), and each appear to make essential contributions to IkB phosphorylation. IKK-i is a serine/threonine kinase that shares homology with IKKaand IKKb. IKK-i is pri-marily expressed in immune cells and is induced by lipopolysaccharide and by proinflammatory cytokines including TNFa, IL-1 and IL-6. Overexpression of IKK-i was shown to result in phosphorylation of IkBaon Ser32 and Ser36, and in NFkB activation, suggesting that IKK-i may act as an IkB kinase in the immune system.\n\nApplications:\nWestern Bloting: 1:500-1:2000.\nELISA: Suggested dilution 1:10000.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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