Ikk alpha, beta (IkappaB Kinase alpha, beta, IKKa,b)

The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB proteins. Most agents that activate NF-kappaB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkappaB. The key regulatory step in this pathway involves activation of a high molecular weight IkappaB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKalpha and IKKbeta serve as the catalytic subunit of the kinase. IKKgamma serves as its regulatory subunit.. Activation of IKK depends on phosphorylation, and the serines 177 and 181 in the activation loop of IKKbeta (176 and 180 in IKKalpha) are the specific sites whose phosphorylation causes a conformational change that results in kinase activation.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA:1:20,000\nWestern Blot:1:500-1:1,000\nImmunohistochemistry:1:50-1:100 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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