IKK alpha (IkappaB Kinase alpha, IKKa, IKK1, IkB Kinase)

The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB proteins. Most agents that activate NF-kappaB do so through a common pathway based on phosphorylation- induced, proteasome-mediated degradation of IkappaB. The key regulatory step in this pathway involves activation of a high molecular weight IkappaB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKK alpha and IKK beta serve as the catalytic subunit of the kinase. IKK gamma serves as it regulatory subunit. Activation of IKK depends on phosphorylation. The serines 177 and 181 in the activation loop of IKK beta (176 and 180 in IKK alpha) are the specific sites whose phosphorylation causes a conformational change that results in kinase activation.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:500\nImmunocytochemistry (ABC): 1:200\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I3000-02

Size

100ul

Applications

ELISA, ICC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Baeuerle, P.A., et al., Science 242: 540-546 (1988). 2. Beg, A.A., et al., Genes Dev. 7: 2064-2070 (1993). 3. Finco, T.S., et al., PNAS USA 91: 11884-11888 (1994). 4.

Additional Info

Detects total IKK alpha proteins. Detects IKK alpha only. Does not crossreact with IKK beta or IKK gamma. Species crossreactivity: Human, mouse, and rat. Predicted to crossreact with bovine based on sequence homology.