IKK-beta (IkappaB Kinase beta, IKKb, IKK2)

IKK-beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK-beta has been shown to activate NF-kB and phosphorylate IKB-alpha and beta (3). Phosphorylation of 2 sites at the activation loop of IKK-beta is essential for activation of IKK by TNF and IL1. Once activated, IKK-beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response (4). Additionally, IKK-beta activity can also be regulated by MEKK-1 (5).\n\nApplications: \nSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:40\nWestern Blot: 1:5,000\nImmunohistochemistry: 1:50-100\nImmunoprecipitation: 1:50\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

I3000-08C

Size

100ul

Applications

FC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Zandi, E.; Rothwarf, D. M.; Delhase, M.; Hayakawa, M.; Karin, M.: Cell 91: 243-252, 1997 2. Scheidereit, C. Docking IB kinase. Nature 395: 225-226, 1998 3. Woronicz, J. D.; Gao, X.; Cao, Z.; Rothe, M.; Goeddel, D. V.: Science 278: 866-869, 1997.4. Delhase, M.; Hayakawa, M.; Chen, Y.; Karin, M.: Science 284: 309-312, 1999. 5. Nakano, H., M. Shindo, S. Sakon, S. Nishinaka, M. Mihara, H. Yagita, and K. Okumura. Proc. Natl. Acad. Sci. USA 95: 3537-3542, 1998

Additional Info

Recognizes human IKK-beta