IKK Isoform Antibody Sampler Kit, BioAssay(TM)

The IKK Isoform Antibody Sampler Kit provides an economical means to investigate NFkB signaling within the cell. The kit contains primary and secondary antibodies to perform four Western mini-blots with each antibody. \n\nThe NFkB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkB proteins (1-3). Most agents that activate NFkB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IkB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKa and IKKb serve as the catalytic subunits of the kinase. IKKg serves as the regulatory subunit (8,9). Activation of IKK depends on phosphorylation; Ser177 and Ser181 in the activation loop of IKKb (176 and 180 in IKKa) are the specific sites whose phosphorylation causes conformational changes resulting in kinase activation (10-13).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I3001

Size

4x40ul

Applications

WB

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

Purity

Purified by protein A and peptide affinity chromatography.

Isotype

IgG

References

(1) Baeuerle, P.A. et al. (1988) Science 242, 540

Additional Info

Detect endogenous levels of total IKKa, IKKb, IKKg and IKKe proteins, respectively. These antibodies do not cross-react with IKK subunits other than their specified target.