IL-10 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human IL-10 in cell culture supernates, serum and plasma (heparin, EDTA, citrate.\n\nInterleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production.1 IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions.2 It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system.3 IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8.4 Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility.5 With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease.6 The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.7 The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6kD.\n\nAssay Range:\n7.8pg/ml-500pg/ml(body fluids, tissue lysates or cell culture supernates)\n3.4pg/ml-250pg/ml(human sera, plasma)\n\n(body fluids, tissue lysates or cell culture supernates)\n3.4pg/ml-250pg/ml \n(human serum, plasma).\n\n\nSensitivity:\n< 0.5 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP22301\n\nEntrez Gene ID:\n3586\n\nKit Components:\n96-well plate pre-coated with anti-IL-10, Human\nLyophilized recombinant IL-10, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143888

Size

96Tests

References

1. Alamartine, E.; Berthoux, P.; Mariat, C.; Cambazard, F.; Berthoux, F. Interleukin-10 promoter polymorphisms and susceptibility to skin squamous cell carcinoma after renal transplantation. J. Invest. Derm. 120: 99-103, 2003. \n2. Grove, J.; Daly, A. K.; Bassendine, M. F.; Gilvarry, E.; Day, C. P. Interleukin 10 promoter region polymorphisms and susceptibility to advanced alcoholic liver disease. Gut 46: 540-545, 2000.\n3. Eskdale, J.; Kube, D.; Tesch, H.; Gallagher, G. Mapping of the human IL10 gene and further characterization of the 5-prime flanking sequence. Immunogenetics 46: 120-128, 1997. \n4. Gesser, B.; Leffers, H.; Jinquan, T.; Vestergaard, C.; Kirstein, N.; Sindet-Pedersen, S.; Jensen, S. L.; Thestrup-Pedersen, K.; Larsen, C. G. Identification of functional domains on human interleukin 10. Proc. Nat. Acad. Sci. 94: 14620-14625, 1997. \n5. Asadullah, K.; Eskdale, J.; Wiese, A.; Gallagher, G.; Friedrich, M.; Sterry, W. Interleukin-10 promoter polymorphism in psoriasis. J. Invest. Derm. 116: 975-978, 2001. \n6. Opdal, S. H.; Opstad, A.; Vege, A.; Rognum, T. O. IL-10 gene polymorphisms are associated with infectious cause of sudden infant death. Hum. Immun. 64: 1183-1189, 2003. \n7. Kim, J. M.; Brannan, C. I.; Copeland, N. G.; Jenkins, N. A.; Khan, T. A.; Moore, K. W. Structure of the mouse Il-10 gene and chromosomal localization of the mouse and human genes. J. Immun. 148: 3618-3623, 1992. \n