IL-10 (IL10)

Interleukin (IL)-10 is a cytokine that inhibits production of other regulatory factors, including interferon gamma (IFN-gamma) and IL-2. A dimer of IL-10 is present in solution and is presumed to participate in receptor binding (1). IL-10 is a natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. Human IL-10 (hIL-10) has high homology with murine IL-10 (mIL-10) as well as with an Epstein-Barr virus genome product BCRFI (2). IL-10 displays pleiotropic immunoregulatory properties. Human and murine IL-10 have a high amino acid sequence identity (ca. 73%) which includes the conservation of all four cysteine residues in human IL-10 and the first four out of five cysteine residues for murine IL-10. IL-10 is a highly alpha-helical protein containing two disulfide bonds, either one or both of which are critical for its structure and function (3).\n\nApplications:\nSuitable for use in Western Blot, Immunocytochemistry, Flow cytometry and Immunoprecipitation. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern Blot: 1:1000-1:10,000 \nImmunoprecipitation: 1:10-1:100 \nImmunocytochemistry: 1:50-1:100\nFlow cytometry: 1:100-1:500\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

I8432-25N

Size

100ul

Applications

FC, ICC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

References

1. Windsor WT, et al. Biochemistry 32(34):8807-15, 1993. 2. Gesser B, et al. Proc Natl Acad Sci 94(26):14620-5, 1997. 3. Zdanov A, et al. Structure 3(6):591-601, 1995.

Additional Info

Recognizes human IL-10.