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IL-12, Mouse, BioAssay(TM) ELISpot Development Module (Interleukin 12, IL-23 p40, CLMF2, IL12B, IL-12B, NKSF2)

Cat no: 145746

IL-12, Mouse, BioAssay(TM) ELISpot Development Module (Interleukin 12, IL-23 p40, CLMF2, IL12B, IL-12B, NKSF2)

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40kD (p40) subunit and a 35kD (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.\n\nSample Type:\nWhole Cells\n\nIntended Use:\nBioAssay(TM) complete ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. Complete ELISpot kits are ready-to-run and require no assay development or refinement. ELISpot Development Modules contain the basic components required to develop an ELISpot assay. \n\nTest Principle:\nThe analyte-specific ELISpot Development Modules contain the components for analyte detection and visualization. Each module contains enough reagents for at least five 96-well microplates.\n\nKit Components:\nMouse IL-12/IL-23 p40 Capture Antibody Concentrate, 1x vial \nMouse IL-12/IL-23 p40 Detection Antibody Concentrate, 1x vial\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C for up to six months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

145746

Size

1Kit

Read more on Supplier website

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

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