IL-13 BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse IL-13 in cell culture supernates, serum and plasma (heparin, EDTA).\n\nInterleukin (IL)-13 is a major inducer of fibrosis in many chronic infectious and autoimmune diseases.1 Recombinant IL-13 protein inhibits inflammatory cytokine production induced by lipopolysaccharide in human peripheral blood monocytes. Moreover, it synergizes with IL-13 in regulating interferon-gamma synthesis in large granular lymphocytes. Interleukin-13 may be critical in regulating inflammatory and immune responses.2 IL-13 and IL-4 are two cytokines produced by T helper type 2 cells, mast cells, and basophils. In addition to their physiological roles, these cytokines are also implicated in pathological conditions such as asthma and allergy.3 The genes encoding human IL-4 and IL-13 are located on segment q23-31 of chromosome 5.4 Recombinant mouse IL-13 is a mixture of Ser26-Phe131 amino acids with the molecular mass of 11.5kD.\n\nAssay Range:\n15.6pg/ml-1000pg/ml\n\nSensitivity:\n< 2 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP20109\n\nEntrez Gene ID:\n16163\n\nKit Components:\n96-well plate pre-coated with anti-IL-13, Mouse\nLyophilized recombinant IL-13, Mouse standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143896

Size

96Tests

References

1. Fichtner-Feigl, S.; Strober, W.; Kawakami, K.; Puri, R. K.; Kitani, A. IL-13 signaling through the IL-13-alpha-2 receptor is involved in induction of TGF-beta-1 production and fibrosis. Nature Med. 12: 99-106, 2006. \n2. Minty, A.; Chalon, P.; Derocq, J.-M.; Dumont, X.; Guillemot, J.-C.; Kaghad, M.; Labit, C.; Leplatois, P.; Liauzun, P.; Miloux, B.; Minty, C.; Casellas, P.; Loison, G.; Lupker, J.; Shire, D.; Ferrara, P.; Caput, D. Interleukin-13 is a new human lymphokine regulating inflammatory and immune responses. Nature 362: 248-250, 1993.\n3. Kelly-Welch, A. E.; Hanson, E. M.; Boothby, M. R.; Keegan, A. D. Interleukin-4 and interleukin-13 signaling connections maps. Science 300: 1527-1528, 2003. \n4. Smirnov, D. V.; Smirnova, M. G.; Korobko, V. G.; Frolova, E. I. Tandem arrangement of human genes for interleukin-4 and interleukin-13: resemblance in their organization. Gene 155: 277-281, 1995. \n