IL-15 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human IL-15 in cell culture supernates, serum and plasma (heparin, EDTA).\n\nInterleukin (IL)-15 is a cytokine with the ability to stimulate the proliferation activity of Th1 and/or Th2 lymphocytes. IL-15 is a novel cytokine whose effects on T-cell activation and proliferation are similar to those of interleukin-2 (IL-2), presumably because IL-15 utilizes the beta and gamma chains of the IL-2 receptor.1 IL-15 can play a role in the initiation and outcome of acute and chronic rejection. Anti-IL-15 therapy in combination with classic immunosuppression therapy might thus be beneficial in the prevention of acute, and especially chronic, allograft rejection.2 The human IL15 gene is mapped to human chromosome 4q31 by fluorescence in situ hybridization.1 The standard product used in this kit is recombinant human IL-15, consisting of 114 amino acids with the molecular mass of 12.9kD.\n\nAssay Range:\n15.6pg/ml-1000pg/ml\n\nSensitivity:\n< 3 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP40933\n\nEntrez Gene ID:\n3600\n\nKit Components:\n96-well plate pre-coated with anti-IL-15, Human\nLyophilized recombinant IL-15, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143897

Size

96Tests

References

1. Anderson, D. M.; Johnson, L.; Glaccum, M. B.; Copeland, N. G.; Gilbert, D. J.; Jenkins, N. A.; Valentine, V.; Kirstein, M. N.; Shapiro, D. N.; Morris, S. W.; Grabstein, K.; Cosman, D. Chromosomal assignment and genomic structure of IL15. Genomics 25: 701-706, 1995. \n2. Conti F, Frappier J, Dharancy S, Chereau C, Houssin D, Weill B, Calmus Y. Interleukin-15 production during liver allograft rejection in humans. Transplantation. 2003 Jul 15;76(1):210-6. \n