IL-15 Mouse BioAssay(TM) ELISA Kit

Murine IL-15 ELISA Cytokine Development kit contains the key components required for the quantitative measurement of natural and/or recombinant Murine IL-15 in a sandwich ELISA format. Using the ELISA protocol described below, the recommended microplates, reagents and solutions, the components supplied in this kit are sufficient to assay Murine IL-15 in approximately 1000 ELISA plate wells.\n\nDetection Range:\nXXX\n\nCross Reactivity:\nYYY\n\nReconstitution and Storage:\n\nCapture Antibody: \n50ug of antigen-affinity purified rabbit anti-Murine IL-15 containing 2.5mg D-mannitol.\nCentrifuge vial prior to opening. Reconstitute in 0.5ml sterile water for a concentration of 100ug/ml.\n?Detection Antibody: \n50ug of biotinylated antigen-affinity purified rabbit anti-Murine IL-15 containing 2.5mg D-mannitol.\nCentrifuge vial prior to opening. Reconstitute in 0.5ml sterile water for a concentration of 100ug/ml.?\nMurine IL-15 Standard: \n1ug of recombinant Murine IL-15 containing 2.2mg BSA, 11.0mg D-mannitol.\nCentrifuge vial prior to opening. Reconstitute in 1ml sterile water for a concentration of 1ug/ml.\n?Note:\nThe reconstituted components are stable for 2 weeks when stored at 2-8 degrees C.\nComponents that have been reconstituted and aliquoted can be stored at -20 degrees C for up to 6 months.\n?Avidin-HRP Conjugate: \n60ul vial. Upon receipt, Avidin-HRP conjugate should be aliquoted into ten 6ul vials and?stored at -20 degrees C or lower. Aliquots stored frozen at below -20 degrees C are stable for up to 2 years from date of receipt. Avoid more than one freeze-thaw cycle. Avidin should be used in conjunction with ABTS only.\n\nBuffer Kit Components:?ELISA microplates, 10x96 well\nSealing Film\nPBS\nWash Buffer\nBlocking Buffer\nDiluent\nABTS Liquid Substrate\n\nExpiration Date:\nOne year from date of receipt\n\nNote:\nAdditional buffers can be purchased with our ELISA Buffer Kit: Cat.# 145859

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