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IL-16 BioAssay(TM) ELISA Kit, Human

Cat no: 143898

IL-16 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human IL-16 in cell culture supernates, serum and plasma (heparin, EDTA).\n\nInterleukin 16 (IL-16) is a cytokine that released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. By Southern blot analysis and PCR using a human/rodent somatic cell hybrid mapping panel, The human IL16 is encoded by a single-copy gene on chromosome 15. Using a combination of STS-content mapping, radiation-hybrid mapping, and genetic mapping, it was refined the assignment to 15q26.1. The mouse Il16 gene was mapped to chromosome 7 in a region showing homology of synteny to human 15q26.1. IL-16 was originally described as a factor that could attract activated T cells in humans, it was previously called lymphocyte chemoattractant factor (LCF), and the augmentation of IL16stimulation by CCR5 plays a role in regulation of Th1 cell recruitment and activation at sites of inflammation.\n\nAssay Range:\n31.2pg/ml-2000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nQ14005\n\nEntrez Gene ID:\n3603\n\nKit Components:\n96-well plate pre-coated with anti-IL-16, Human\nLyophilized recombinant IL-16, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143898

Size

96Tests

References

1. Cruikshank W, Center DM (1982). \"Modulation of lymphocyte migration by human lymphokines. II. Purification of a lymphotactic factor (LCF)\".J. Immunol. 128 (6): 2569

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