IL-17A/F, Recombinant, Human (Interleukin-17A/F Heterodimer) (Carrier free)

IL-17A/F is a cystine-linked heterodimer of IL-17A and IL-17F (1,2). IL-17A/F is produced by Th17 cells, and y8 T cells (1,2). IL-17A/F stimulates the production of pro-inflammatory cytokines and neutrophil chemoattractants, thereby functioning as a bridge between adaptive and innate immunity (1-3). Some research studies suggest that IL-17A/F may be involved in mucosal immunity against some bacterial infections and has a putative role in some autoimmune disorders (3,4). The receptor for IL-17A/F consists of a heterodimer of IL-17RA and IL-17RC and signaling through this receptor leads to the activation of the Erk1/2 and NF-kB pathways (1).\n\nSource:\nRecombinant protein corresponding to human IL-17A/F, expressed in human 293 cells.\n\nMolecular Weight: \n~31.396kD\n\nBiological Activity:\nDetermined by its ability to induce mouse IL-6 production by 3T3 MEFs WT. The ED50 of each lot is 50-200ng/ml.\n\nEndotoxin:\n<0.01ng/1ug \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

137448

Size

10ug

Form

Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute in sterile PBS.

Purity

~98% (SDS-PAGE)

References

1. Iwakura, Y. et al. (2011) Immunity 34, 149-62. 2. Chang, S.H. and Dong, C. (2007) Cell Res 17, 435-40. 3. Henry, T. et al. (2010) J Immunol 184, 3755-67. 4. Hu, Y. et al. (2010) J Immunol 184, 4307-16.