IL-21 BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse IL-21 in cell culture supernates and serum.\n\nInterleukin-21 is a protein that in humans is encoded by the IL21 gene. Interleukin 21 is a cytokine that has potent regulatory effects on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. By radiation hybrid analysis, the IL21 gene is mapped to chromosome 4q26-q27, near the IL2 gene. IL21 stimulates B-cell proliferation in an anti-CD40-dependent manner but inhibits proliferation stimulated by IL4 plus anti-IgM. The biologic effects of IL21 on NK cells differ between species, with human IL21 enhancing NK cell proliferation and murine IL21 inhibiting NK cell proliferation. IL21 is a cytokine highly expressed by mouse T(H)17 cells.\n\nAssay Range:\n62.5pg/ml-4000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nQ5SUE2\n\nEntrez Gene ID:\n60505\n\nKit Components:\n96-well plate pre-coated with anti-IL-21, Mouse\nLyophilized recombinant IL-21, Mouse standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143910

Size

96Tests

References

1. Mehta, D. S., Wurster, A. L., Whitters, M. J., Young, D. A., Collins, M., Grusby, M. J. IL-21 induces the apoptosis of resting and activated primary B cells. J. Immun. 170: 4111-4118, 2003.\n2. Nurieva, R., Yang, X. O., Martinez, G., Zhang, Y., Panopoulos, A. D., Ma, L., Schluns, K., Tian, Q., Watowich, S. S., Jetten, A. M., Dong, C. Essential autocrine regulation by IL-21 in the generation of inflammatory T cells. Nature 448: 480-483, 2007.\n3. Parrish-Novak, J., Dillon, S. R., Nelson, A., Hammond, A., Sprecher, C., Gross, J. A., Johnston, J., Madden, K., Xu, W., West, J., Schrader, S., Burkhead, S., and 26 others. Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. Nature 408: 57-63, 2000.\n