IL-21, Recombinant, Mouse

IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation.Its action generally enhances antigen-specific responses of immune cells.The biological effects of IL-21 include induction of differentiation of T-cells-stimulated-B-cells into plasma cells and memory B-cells, stimulation (in conjunction) with IL-4 of IgG production, and induction of apoptotic effects in naiumlve B-cells and stimulated B-cells in the absence of T-cell signaling.Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells.IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (gammac) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15.The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.Recombinant murine IL-21 is a 15.0kD protein consisting of 130 amino acid residues.\n\nBiological Activity:\nMeasured by its ability to increase proliferation in mouse splenocytes induced by anti-hCD40 mAb. The expected ED50 is 15-20 ng/ml.\n\nAA Sequence:\nMHKSSPQGPDRLLIRLRHLIDIVEQLKIYENDLDPELLSAPQDVKGHCEHAAFACFQKAKLKPSNPGNNKTFIIDLVAQLRRRLPARRGGKKQKHIAKCPSCDSYEKRTPKEFLERLKWLLQKMIHQHLS\n\nQuality Control:\nVerified by N-terminal and Mass Spectrometry analyses (when applicable).\n\nEndotoxin:\n<0.1ng/ug of protein (<1EU/ug).\n\nProtein Content:\nVerified by UV Spectroscopy and/or SDS-PAGE gel.\n\nStorage and Stability:\nStore lyophilized products at -20 degrees C. For reconstituted solutions of most products, we recommend short-term storage at 4 degrees C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20 degrees C. Additional freeze/thaw cycles may cause some denaturation of the protein.

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